Jacqueline de Rohan, Marquise de Rothelin (c. 1520 – 1587) was the daughter of Charles de Rohan and Jeanne de Saint-Severin.
Her paternal grandparents were Pierre de Rohan, Viscount de Fronsac and Françoise de Penhoet. Her maternal grandparents were Bernard de Saint-Severin, Prince of Besignano, and Jeanne Eléonore Piccolomini. The latter was a descendant of King Alfonso V of Aragon and his mistress Giraldona Carlino.
On 19 June 1536, at Lyon, she married François of Orléans-Longueville, Marquis de Rothelin, Prince of Chalet-Aillon, Viscount of Melun (2 March 1513 – 25 October 1548), son of Louis I d'Orléans, duc de Longueville, Duke of Neufchatel, Prince of Chatel-Aillon and Johanna of Baden-Hochberg, Countess of Neufchatel and Margravine of Rothelin, by whom she had two children:
Her husband died on 25 October 1548, and in watching her son Leonor's interests in Neuchâtel she was brought into contact with the reformers in Switzerland. She then embraced Protestantism and turned her château at Blandy, in Brie, into a refuge for Huguenots.
In 1567 she underwent a term of imprisonment at the Louvre for harbouring Protestants.